What Is DNA Fingerprinting

Q.

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

1. Annealing

2. Extension

3. Ligation

4. Denaturation

Q.

Satellite DNA is important because it

1. Codes for enzymes needed for DNA replication

2. Does not code for proteins and is same in all members of the population

3. Shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children

4. Codes for proteins needed in cell cycle

Q.

What is DNA fingerprinting? Mention its application.

Q.

List The Advances In Technology That Have Improved Forensic Analysis

Q. Primers used for the process of polymerase chain reactions are

1. double-stranded DNA oligonucleotide
2. single-stranded RNA nucleotide
3. single-stranded DNA nucleotide
4. double-stranded DNA oligonucleotide

Q.

What is expressed sequence tags?

Q. Pick the incorrect statements among the following.
(i) PCR is one of the techniques used in DNA fingerprinting.
(ii) PCR can be used for detection of a specific mutation or pathogen.
(iii) Alec Jeffreys won the Nobel Prize for the development of polymerase chain reaction (PCR).
(iv) DNA primers are designed such that they can attach anywhere on the DNA segment which has to be amplified.

1. i, iv
2. i, ii, iii
3. i, ii, iv
4. iii, iv

Q. What is DNA fingerprinting? Mention its application.

Q. Size of VNTR varies from

1. 0.01 to 20kb
2. 0.1 to 20kb
3. 0.01 to 10 bp
4. 0.1 to 10 bp

Q. Length of one coil of B-DNA helix is

1. 0.4 nm
2. 3.4 nm
3. 10 nm
4. 20 nm

Q.

Which Cannot Be Used For DNA Fingerprinting In Humans?

Q. Arrange the following steps of DNA fingerprinting in their correct sequence.

1. Autoradiography
2. Isolation of DNA
3. Southern blotting
4. Cleavage by restriction endonucleases
5. Gel electrophoresis

1. I, II, IV, V, III
2. II, V, III, IV, I
3. II, IV, V, III, I
4. V, II, III, IV, I

Q. A recombinant DNA molecule can be produced in the absence of the following

1. DNA ligase
2. Restriction endonuclease
3. DNA Fragments
4. E. Coli

Q. [3]
(a) Expand VNTR and describe its role in DNA fingerprinting.

(b) List any two applications of DNA fingerprinting technique.

Q. Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

1. Polymerase chain reaction
2. Zinc finger analysis
3. Restriction enzymes
4. DNA - DNA hybridization

Q.

Process used for amplication or multiplication of DNA for fingerprinting is:

1. Nesslerisation

2. Northern blotting

3. Polymerase chain reaction

4. Southern blotting

Q.

Do twins have the same DNA and fingerprints?

Q.

Fill in the blanks from A to E for the steps involved in rDNA technology.

1. Isolation of DNA from plant cells primarily requires treatment of cells by __A__ enzyme.

2. DNA in gel electrophoresis moves from ___B____ to ___C___.

3. PCR is the procedure to ___D___ DNA.

4. Restriction endonuclease EcoRI was isolated from ___E___.

1. A: Chitinase: B: Anode; C: Cathode; D: Amplify; E: E. coli

2. A: Cellulase: B: Cathode; C: Anode; D: Amplify; E: E. coli

3. A: Chitinase: B: Cathode; C: Anode; D: Separate; E: E. coli

4. A: Cellulase: B: Anode; C: Cathode; D: Isolate; E: E. coli

Q. All are applications of DNA fingerprinting except

1. In forensic science
2. To amplify minisatellite
3. Diagnosis of inherited disorders
4. Paternity testing

Q. Two blood samples A and B were picked up from a crime scene. How would you determine if the samples belong to the same individual or different individuals? Explain your result. [5]

Q. Which of the following is not correct :-

1. Heterogeneous nuclear RNA (hn RNA) are the smallest molecules which carry amino acids to the site of protein synthesis.
2. Temin and Baltimore reported reversal of central dogma, by discovering reverse transcription in some viruses.
3. In transcription only a segment of DNA and only one of the strands is copied into RNA.
4. The coding DNA sequence of the gene are called exons and the intervening non-coding DNA sequences are called introns.

Q. Gene amplification using primers can be done by:

1. Microinjection
2. ELISA
3. Polymerase chain reaction
4. Gene gun

Q.

What are the parameters which are characteristics for DNA extraction procedures?

Q.

Match the following:

$\begin{array}{cc}\text{A. VNTR}& \text{p. Largest}\\ \text{B. Introns and Exons}& \text{q. DNA fingerprinting}\\ \text{C. Dystrophin}& \text{r. Bulk DNA}\\ \text{D. Satellite DNA}& \text{s. Splicing}\end{array}$

1. A – q, B – s, C – p, D – r

2. A – s, B – p, C – q, D – r

3. A – r, B – s, C – p, D - q

4. A – q, B – p, C – s, D – r

Q. What is DNA fingerprinting? Mention its application.

Q. Assertion :DNA fingerprinting involves identifying differences in specific regions in DNA sequence. Reason: DNA fingerprinting is the basis of paternity testing.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
3. Assertion is correct but Reason is incorrect.
4. Both Assertion and Reason are incorrect.

Q. The following figure represents the

1. Central dogma
2. Lac operon
3. Sequence Annotation
4. DNA fingerprinting

Q. is it possible for two siblings having the blood group AB and O one male and another female respectively.

Q.

How much of human DNA is the same?

Q. Arrange the various steps of DNA fingerprinting technique in the correct order
(i) Separation of DNA fragments by electrophoresis
(ii) Digestion of DNA by restriction endonucleases
(iii) Hybridization using labeled VNTR probe
(iv) Isolation of DNA
(v) Detection of hybridized DNA fragments by autoradiography
(vi) Transferring the separated DNA fragments to nitrocellulose membrane

1. (ii) $\to$ (i) $\to$ (iv) $\to$ (vi) $\to$ (iii) $\to$ (v)
   
2. (iv) $\to$ (i) $\to$ (ii) $\to$ (iii) $\to$ (vi) $\to$ (v)
3. (iii) $\to$ (v) $\to$ (iv) $\to$ (ii) $\to$ (i) $\to$ (vi)
4. (iv) $\to$ (ii) $\to$ (i) $\to$ (vi) $\to$ (iii) $\to$ (v)