A person suspected to suffer from AIDS is advised to undergo ............ diagnostic test.
A
Ultrasound
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B
WIDAL
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C
MRI
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D
ELISA
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Solution
The correct option is D ELISA
ELISA (Enzyme-linked immunosorbent assay) is used as a diagnostic test for identification of HIV virus. The assay uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured.
In ELISA, antigens from the sample are attached to a surface. Then, a matching antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a colour change in the substrate.