Explant develops into callus followed by-

The correct option is D

All of the above

The correct option is D.

Explanation of the correct option:

1. Explant refers to a small part of the plant which has the potential and is capable of regenerating into a whole plant.
2. When an explant is provided with the required medium, all the essential hormones as well as the sterile condition it grows into a callous which is nothing but a piece of unorganized cells that can later regenerate into the entire plant.
3. Explants are immersed in a suitable concentration of chemical sterilants or disinfectants for a certain amount of time during the surface sterilization procedure, which produces a culture that is free of contamination.
4. The polysaccharide agar, which is derived from seaweeds, is widely used as a gelling agent to create semi-solid and solid plant tissue culture media.
5. A growth medium, also known as a culture medium, is a solution or gel that promotes the development of cells, tiny plants, or microbes.
6. The majority of cell culture mediums contain substances that control the cell cycle and are a suitable source of energy.

Final answer: Explant develops into callus followed by sterile conditions, growth-supporting medium, and hormone supplementation.