In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. In bacterial transformation, a scientist uses restriction enzymes EcoRI and HaeIII in a digest of a bacterial plasmid. The resulting DNA fragments are shown in the gel electrophoresis below.
What is the total size of the plasmid?

Q. Consider the following steps during recombinant DNA technology.
a. Breaking of donor DNA using restriction enzyme
b. Culture of cloned bacteria containing the fragment of donor DNA
c. Isolation of suitable plasmid
d. Insertion of recombinant DNA through cloning
e. Joining of donor DNA with a suitable plasmid using ligase
The correct sequence of these is?

Q. The different steps of recombinant DNA technology are given below randomly.(i) Isolation of the DNA fragments or genes to be cloned(ii) Introduction of the recombinant DNA into a suitable cell (usually E.coli) called host (transformation)(iii) Multiplication/expression of the introduced gene in the host(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment(v) Insertion of the isolated gene in a suitable plasmid vectorWhich of the following represents the correct sequence of steps ?