Name the source from which insulin was extracted earlier. Why is this insulin no more is use by diabetic people?

Q. Renuka's father is suffering from diabetes and is dependent on insulin for diabetes treatment. Earlier it was obtained from animals and was costly but now it is cheaper and easily available. it is due to biotechnology.
Read the above passage and answer the followings questions:
What is the role of insulin?

Q. Which of the following statement(s) is/are incorrect?
(i) Insulin was originally extracted from the pancreas of slaughtered pigs and cattle.
(ii) Animal insulin is identical to human insulin.
(iii) Non-human insulin causes some patients to develop allergies.
(iv) Recombinant insulin is actually obtained from E. coli in bacterial cells.

Q. Insulin is extracted from which microorganism?