Separation of chromatids during metaphase and behaviour of chromosomes during mitotic and meiotic divisions can be best studied by

The correct option is A Phase contrast microscope
Phase contrast microscope has a phase plate and an annular diaphragm. They bring about changes in light rays passing through the specimen producing differences in light intensity. Denser parts alter path of light more than the thinner parts. This produces varying contrast for different regions.

The microscope is useful in the study of small living organisms, living cells, constituents of living cells, effect of various factors over living structures, and study of various cellular events like behaviour of chromosomes during mitosis and meiosis, spindle formation, cytokinesis, pinocytosis, phagocytosis, cyclosis, glandular secretions, spermatogenesis, oogenesis etc.