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Question

The correct palindromic sequence of the restriction enzyme, Hind III, is:


A

AAGCTTTTCGAA

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B

GAATTCCTTAAG

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C

GATATCCTATAG

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D

GGATCCCCTAGG

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Solution

The correct option is A

AAGCTTTTCGAA


The correct option is option A.

Explanation of the correct option:

  1. It is the restriction site of the enzyme Hind III.
  2. It is palindromic in nature.
  3. It cleaves the sequence AAGCTT between the AA in presence of Mg+2 to give 5' overhangs, also called sticky ends.
  4. This enzyme is present in Haemophilus influenza hence the name Hind III.

Explanation of the incorrect options:

Option B:

  1. It is the restriction site of the enzyme EcoRI.
  2. This enzyme is present in the bacteria Escherichia coli strain RY.

Option C:

  1. It is the restriction site of the enzyme EcoRV.
  2. It is present in certain strains of the bacteria Escherichia coli.

Option D:

  1. It is the restriction site of the enzyme BamHI.
  2. It is present in the bacteria Bacillus amyloliquefaciens.

Final answer: The correct palindromic sequence of the restriction enzyme, Hind III, is AAGCTTTTCGAA.


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