Which restriction enzymes are used for selectabmarker tet on pBR 322(1) PvuI, Pst(3) Sal I, Bam HI(2) Pvu l, Bam Hl(4) Pst I, Sal I

Q. Plasmid pBR322 has Pst I restriction enzyme site within gene amp^R that confers ampicillin resistance. If this enzyme is used for inserting a gene for ß-galactoside production and the recombinant plasmid is inserted in an E. coli strain

Q. Which among the following restriction endonucleases will produce blunt ends?
I. BamH I
II. Hind II
III. EcoR I
IV. Hind III
V. Sal I

Q. In a pBR 322, a foreign DNA segment has to be introduced in the $te{t}^R$ region. From the restriction enzymes, $BamHI$ and $EcoRI$, which one would you select for use? Give reason. Also mention why not the other one.

Q. What error are you likely to get when you run the following program?
main()
{
struct emp;
{
char name [20];
float sal;
}
struct emp e[10];
int i;
for (i=0;i<=9;i++)
scanf("%s%f",e[i].name&e.[i].sal));
}