ELISA

Q. AIDS is characterized by

1. decrease in the number of killer T-cells
2. decrease in the number of supressor T-cells
3. decrease in the number of helper T-cells
4. increase in the number of helper T-cells

Q. ELISA is used to detect viruses where the key reagent is:

1. Alkaline phosphatase
2. Catalase
3. DNA probe
4. RNase

Q. What are limitations of ELISA test?

Q. ELISA can give a false negative result in the beginning of an infection due to

1. low DNA concentration in the sample
2. high concentration of pathogen
3. low concentration of pathogen
4. low RNA concentration in the sample

Q. Human immunodeficiency virus (HIV) has a protein coat and a genetic material which is

1. Single stranded DNA
   
2. Double stranded RNA
   
3. Double stranded DNA
   
4. Single stranded RNA
   

Q.

What is the sandwich ELISA?

Q.

Mention one function of the selectable markers.

Q. ELISA test is used for the detection of

1. hepatitis, tuberculosis and AIDS
2. AIDS, STD.and hepatitis
3. typhoid, hepatitis and AIDS
4. STD, tuberculosis and AIDS

Q. Assertion :ELISA test is based on antigen-antibody interactions where a pathogen can be detected by the presence of antibodies (proteins, glycoproteins, etc.) on it. Reason: The pathogen antibody to be identified is immibilised on the surface of specially constructed ELISA plares and is then tested.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Name the test which is based on the principle of the antigen-antibody interaction.

Q. On what principle ELISA test is based?

Q. ELISA test is used to

1. Separate viral RNA
2. Purify proteins
3. Isolate DNA sequences
4. Identify specific proteins

Q. ELISA is

1. Enzyme linked immunosorbent assay
2. Energy linked immunosorbent assessment
3. Enzyme linked immunosorbent array
4. Enzyme ligated immunosorbent assay

Q. Match the images and the steps of ELISA

a. A substrate is present which the enzyme cleaves and colored reaction is produced
b. Enzyme linked antibody binds to specific antibody.
c. Blood serum from the patient is added and incubated for 30 minutes(wash)
​d. The well is first coated with a antigen and then washed

1. 
2. 
3. 
4. 

Q. Expand ELISA. On what principle is ELISA test based? List two ways by which an infection can be detected by this test.

Q. What is meant by ELISA test?

Q. ELISA is based on

1. probe binding
2. hybridization
3. sequence of amino acids in a protein
4. Antigen antibody interaction

Q. Expand ELISA.

1. Enzyme linked inductive assay
2. Enzyme linked immunosorbent assay
3. None of the above
4. Enzyme linked ion sorbent assay

Q. ELISA is used to detect viruses where the key reagent is

1. DNA probe
2. Alkaline phosphatase
3. Catalase
4. RNase

Q. The competitive indirect ELISA differs from direct ELISA, as in competitive indirect ELISA

1. Unlabeled antibody is incubated in the presence of its antigen.
2. Labelled antibody is incubated in the presence of its antigen.
3. Labelled antigen is incubated in the presence of its antibody.
4. None of the above.

Q. 'ELISA' test is done for the diagnosis of

1. Anthrax
2. HIV
3. Hepatitis
4. Malaria

Q. Which one of the following techniques is used for the detection of proteins ?

1. Nothern blotting
2. Southern blotting
3. In-situ hybridization
4. Western blotting

Q. Name a popular recent technique, which is used for detecting the presence of pathogen/virus in an organism.

Q. A patient is suspected to be suffering from Acquired ImmunoDeficiency Syndrome (AIDS). Which diagnostic technique will you recommend for its detection ?

1. Ultrasound
2. Widal
3. ELISA
4. MRI

Q. AIDS is characterized by

1. decrease in the number of killer T-cells
2. decrease in the number of supressor T-cells
3. decrease in the number of helper T-cells
4. increase in the number of helper T-cells

Q. Which of the following criteria must be considered while selecting enzyme for the ELISA?

1. Purity
2. Sensitivity
3. Stability
4. All of the above

Q. ELISA is

1. Energy linked immunosorbent assessment
2. Enzyme linked immunosorbent assay
3. Enzyme linked immunosorbent array
4. Enzyme ligated immunosorbent assay

Q. A person suspected to suffer from AIDS is advised to undergo ............ diagnostic test.

1. Ultrasound
2. WIDAL
3. MRI
4. ELISA

Q. Expand and mention one application of ELISA.

Q. Which of the following statements can be related to ELISA?

1. It uses complement mediated cell lysis.
2. It uses radiolabelled second antibody.
3. It involves addition of substrate which is converted into colored end product.
4. It requires RBC's.