Gene Therapy

Q.

Which of the following is not a Mendelian disorder?

1. Colour blindness

2. Cystic fibrosis

3. Haemophilia

4. Turner's syndrome

Q. The number of recombinant therapeutics that were marketed in India was ______.

1. 13
2. 14
3. 12
4. 11

Q.

Who is the father of gene therapy?

Q.

What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.

Q.

What is gene therapy? Illustrate using the example of adenosine deaminase (ADA) deficiency.

Q.

As per the recent ethical rules laid down by the National Institute of Health, diseases that cannot be treated $\_\_\_\_\_\_\_\_\_\_$would qualify for treatment involving human gene manipulation

1. Dangerous

2. Alternatively

3. Uncommon

4. Similar

Q. During a murder investigation, blood stains from the crime scene, blood of the victim and 3 suspects were collected. DNA fingerprinting was performed for all of them and the autoradiogram thus obtained has been given below.

Which of the suspects is likely to have committed the crime?

1. Suspect 1
2. Suspect 2
3. Suspect 3
4. Blood stain collected at crime site is of victim

Q. pBR322 is frequently used as a vector in biotechnology. It helps in cloning foreign genes of interest. Which of the following is the correct statement about this vector?

1. It has three antibiotic resistance genes
2. It contains the restriction site for Sal I within the tetracycline resistance gene
3. It occurs naturally in Escherichia coli
4. Both b and c

Q. An example of gene therapy is :

1. Production of injectable hepatitis B vaccine
2. Production of vaccines in food crops like potatoes which can be eaten
   
3. Introduction of gene for adenosine deaminize in persons suffering from severe combined immune deficiency (SCID)
4. Production of test tube babies by artificial insemination of unfertilized eggs.
   

Q. House keeping genes

1. Produce antibodies
2. Constantly operate for cellular activity
3. Form hormones
4. Function only at the time of reproduction.

Q. First gene therapy was performed for the disease:

1. Down’s syndrome
2. ADA deficiency
3. Cystic fibrosis
4. Alkaptonuria

Q. In gene therapy, functional gene is inserted into target cell primarily for

1. Replacing non-functional gene
2. Making desired amount of protein
3. Stopping complete expression of gene
4. Suppressing non-expressible gene

Q. Match column I and column II and choose the correct option.

	Column-I		Column-II
a.	cryIAc	(i)	A. tumefaciens
b.	Taq polymerase	(ii)	Thermus aquaticus
c.	Native plasmid of S. typhimurium	(iii)	Specific Bt toxin gene
d.	Natural genetic engineer for plants	(iv)	First recombinant DNA

1. a - iii, b - iv, c- ii, d - i
2. a - iii, b - ii, c - iv, d - i
3. a - i, b - ii, c - iii, d - iv
4. a - ii, b - iii, c - iv, d - i

Q. Somatic gene therapy is the transfer of gene(s) into _____A_____. It is __B______ exerting its effect on _____C________.

1. A- body cells except the germ cells, B- non-heritable, C- patient only
2. A- germ cells, B- non-heritable, C- patient only
3. A- body cells except the germ cells, B- heritable, C- future generations
4. A- embryo, B- heritable, C- future generations

Q. Ori Site: Initiation of replication :: Antibiotic resistance gene: __________

1. Restriction enzyme recognition site
   
2. Selectable marker
   
3. Repressor gene
   
4. Promoter sequence
   

Q.

In plants, what is Apical dominance?

Q. Introduction of the remedial gene into the cells of the bone marrow falls under _________.

1. Germ line gene therapy
2. Somatic cell gene therapy
3. Both a and b
4. Ex-vivo cell gene therapy

Q. Appearance of antibiotic resistance gene in bacteria is an example of

1. Adaptive radiation
2. Convergent evolution
3. Divergent evolution
4. Pre-existing variation in the population

Q. The most popular vector used in human gene therapy is

1. Plasmid
2. Phage
3. PUC 18
4. Retrovirus

Q.

The genetic defect - Adenosine Deaminase (ADA) deficiency may be cured permanently by

1. administering adenosine deaminase activators

2. periodic infusion of genetically engineered lymphocytes having functional ADA C-DNA

3. introducing bone marrow cells producing ADA into cells at early embryonic stages

4. enzyme replacement therapy

Q. What is gene therapy? Mention the two types of gene therapies.

Q. ADA deficiency results in:

1. Increased risk of infertility
2. Inability of immune system to function normally
3. Chromosomal disorders
4. Decrease in yield of crop plants

Q.

What is gene therapy?

1. A therapy in which all the faulty genes of the body are destroyed.

2. A technique to replace 'a faulty gene' by a normal healthy functional gene.

3. Changing the genetic make up of sperms.

4. None of these.

Q.

1. Amniocentesis
2. Genetic engineering
3. Gene therapy
4. Transgenic organism

Q. Biolistic (gene-gun) is suitable for:

1. Disarming pathogen vector
2. Transformation of plant cells
3. Constructing recombinant DNA by joining with vectors

4. DNA fingerprinting

Q.

What is gene therapy?

1. A therapy in which all the faulty genes of the body are destroyed.

2. None of these.

3. A technique to replace 'a faulty gene' by a normal healthy functional gene.

4. Changing the genetic make up of sperms.

Q. In gene therapy, a functional gene is inserted into:

1. An embryo only
2. Either an individual or an embryo
3. Both an individual and an embryo
4. An individual only

Q.

how is PCR used to detect gene mutations in case of suspected cancer patients?

Q. Arrange the steps of ADA-deficiency treatment in sequence:
I. The lymphocytes with ADA cDNA is returned to the patient
II. The lymphocytes from the blood of the patient are grown in culture outside the body
III. A functional ADA cDNA (using retroviral vector) is introduced into the lymphocytes

1. $II\to I\to III$
2. $III\to II\to I$
3. $I\to III\to II$
4. $I\to II\to III$

Q. Why is gene therapy needed?

1. Gene therapy can be designed to suit your genome and hence is more effective
2. Gene therapy is a better cure for diseases as it has no side effects
3. Genetic disorders cannot be cured by drug based medicines
4. Certain viral diseases need to be combated by gene silencing