Genetic Engineering

Q. The sticky ends facilitates the action of the enzyme __________.

1. DNA Ligase
2. DNA Polymerase
3. restriction endonuclease
4. exonuclease
   

Q.

What is the application of cloning?

Q.

How human insulin is produced from biotechnology? (3 marks/5 marks)

Q. Assertion: Restriction enzymes recognise specific palindromic sequences.
Reason: Palindromic sequences are DNA sequences that are read the same way when the orientation is kept the same in both the strands.

1. Both assertion and reason are true and reason is the correct explanation of assertion
2. Both assertion and reason are true but reason is not the correct explanation of assertion
3. Assertion is true but reason is false
4. Both assertion and reason are false

Q. Which among the following restriction endonucleases will produce blunt ends?
I. BamH I
II. Hind II
III. EcoR I
IV. Hind III
V. Sal I

1. I, IV
2. Only III
3. Only II
4. III, IV

Q. Statement 1: Bt Cotton is a transgenic crop which is resistant to pests.
Statement 2: The gene responsible for providing pest resistance to this GM crop is
obtained from the bacteria Agrobacterium tumefaciens.

1. Both the statements 1 and 2 are correct
2. Both the statements 1 and 2 are incorrect
3. Statement 1 is correct but statement 2 is incorrect
   
4. Statement 1 is incorrect but statement 2 is correct
   

Q.

How to group the DNA manipulative enzymes?

Q. In a mixture, DNA fragments are separated by:

1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction

Q.

What Is humulin?

Q.

What are restriction enzymes? Give examples.

Q. For the commercial and industrial production of citric acid, which of the following microbes is used?

1. Aspergillus niger
2. Lactobacillus sp
3. Saccharomyces cerevisiae
4. Clostridium butylicum

Q.

Who Discovered Transformation?

Q.

Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?

Q. EFB stands for

1. European Federation of Biotechnology
2. Eurasian Federation of Biotechnology
3. East Asia Federation of Biotechnology
4. Ethiopian Federation of Biotechnology

Q. Which of the following is not a feature of the plasmids?

1. Single stranded
2. Circular structure
3. Transferable
4. Independent replication

Q. Which of the given enzymes is capable of opening of DNA helix and polymerisation of nucleotides?

1. DNA polymerase I
2. RNA polymerase
3. DNA polymerase II
4. DNA polymerase III

Q. Read the following statements about asexual reproduction and select the correct ones.

(i) It involves a single parent.
(ii) It is slower than sexual reproduction.
(iii) It produces progeny that are genetically identical with the parent but not with one another.
(iv) The progeny of asexual reproduction can be termed as clones.

1. (i) and (ii)
2. (ii) and (iii)
3. (i) and (iv)
4. (i), (iii) and (iv)

Q. Which among the following restriction endonucleases will produce blunt ends?
I. BamH I
II. Hind II
III. EcoR I
IV. Hind III
V. Sal I

1. I, IV
2. Only III
3. Only II
4. III, IV

Q. When the recognition sequence of two different restriction endonucleases are the same, with the location of the cut within the site also being the same, they are called as

1. Neoschizomers
2. Isoschizomers
3. Isocaudomers
4. Palindromes

Q. In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to

1. Isolate fragments of cloned bacterial plasmids.
2. Isolate fragments of frog DNA that contained an rRNA gene.
3. Cleave the bacterial plasmid.
4. All the above

Q.

Which chemical is used for induction of polyploidy?

1. Cytokinin

2. Colchicine

3. Nitrous acid

4. IAA

Q.

What are interesting facts about cocci?

Q.

Are plasmids found In bacterial cytoplasm?

Q. Which of the following is the product of biotechnology?

1. Transgenic crops (GM crops)
2. Humulin
3. Biofertilizer
4. All of the above

Q. The technique of gel electrophoresis was developed by

1. Arne Tiselius
2. Kary Mullis
3. Nirenberg
4. Alec Jeffreys

Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

1. B $\to$ E$\to$ C $\to$ F $\to$ A $\to$ D $\to$ G
2. B $\to$ F $\to$ D $\to$ A $\to$ C $\to$ E $\to$ G
3. B $\to$ C$\to$ F $\to$ D$\to$ E $\to$ A $\to$ G
4. B $\to$ F $\to$ C $\to$ E $\to$ D $\to$ A $\to$ G

Q. Which of the following is not a restriction endonuclease?

1. Eco RI
2. Hind III
3. DNAse I
4. Pst I

Q.

Advantages of selecting drosophila as an experimental organisms in genetics

Q. Assertion: Term clone is used for the offspring produced by asexual reproduction.
Reason: Offspring produced asexually are morphologically and genetically identical to each other and to the parent.
[1 mark]

1. Assertion and reason are true and the reason is the correct explanation of the assertion
2. Assertion and reason are true but the reason is not the correct explanation of the assertion
3. The assertion is true but the reason is false
4. Both assertion and reason are false

Q.

Restriction endonuclease

1. Synthesizes DNA

2. Cuts the DNA molecule randomly

3. Restricts the synthesis of DNA inside the nucleus

4. Cuts the DNA molecule at specific sites