Ti Plasmid

Q. Which of the following statements regarding Baculoviruses are correct?

1. Baculoviruses are pathogens that attack insects and other arthropods
   
2. Most of Baculoviruses are biocontrol agents belonging to the genus Nucleopolyhedrovirus
3. Baculoviruses do not harm non-target insects
4. All of the above

Q. What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?

Q. In the agrobacterium mediated gene transfer using leaf disc method, what is the significance of cutting the leaf into discs?

1. To make the leaf round shape
2. To induce induce dedifferentiation in leaf cells
3. To increase surface area for transfers
4. To use both surfaces of the leaf

Q. The tumor inducing (Ti) plasmid has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants because Ti plasmid has been modified by :

1. Deleting tumor forming genes
2. Adding tumor forming genes
3. Deleting endonuclease
4. Adding genes resistant to endonucleases

Q. Mutations in plant cells can be induced by

1. Zeatin
2. Kinetin
3. Infrared rays
4. Gamma rays

Q. The proteins encoded by Cry I Ac and Cry II Ab genes control.

1. Boll worms
2. Pin worms
3. Book worms
4. Nematodes

Q. Transgenes are transferred to plants using Ti plasmid present in:

1. Salmonella typhimurium
2. Agrobacterium tumefaciens
3. Bacillus thuringiensis
4. Pseudomonas fluorescens

Q. Bacterial artificial chromosomes (BACs), cosmids, phages, plasmids and yeast artificial chromosomes (YACs) are all commonly used cloning vectors that differ in their cloning capacities, with a range from approximately 100 bp to 1000 kb. Which of the following is the increasing cloning capacity?

1. BAC, cosmid, phage, plasmid, YAC
2. YAC, BAC, cosmid, phage, plasmid
3. Plasmid, Cosmid, phage, BAC, YAC
4. Plasmid, phage, cosmid, BAC, YAC

Q. The bacteria associated with plant genetic engineering are

1. Salmonella and Pseudomonas
2. Salmonella typhimurium and Agrobacterium tumefaciens
3. Bacillus thuringiensis and Pseudomonas fluorescens
4. Rhizobium leguminosarum and Azotobacter agilis

Q. Cry genes or Bt genes are obtained from ?

1. Cotton pest
2. Tobacco pest
3. Bacillus thuringiensis
4. E.coli

Q. Observe the diagram and answer the following questions


AOOTING MEDIUM + KANAMYCIN + KAAAMYCIN + CARBENICILLIN CARBENICILLIN SHOOTS REGENERLATE IN 3-4 WEEKS

Q. Plasmids are vectors for gene cloning because they

1. Self replicate in bacterial cells
2. Replicate freely outside bacterial cells
3. Can be multiplied in culture
4. Can be multiplied in laboratories using enzymes

Q. The Ti-plasmid is used for making transgenic plants. This plasmid is found in

1. Agrobacterium
2. Yeast as a 2µm plasmid
3. Azotobacter
4. Rhizobium of the roots of leguminous plants

Q. Transgenes are transferred to plants using Ti plasmid present in

1. Salmonella typhimurium
2. Bacillus thuringiensis
3. Agrobacterium tumefaciens
4. Pseudomonas fluorescens

Q. In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?

1. To make the leaf round shape
2. To use both surfaces of the leaf
3. To induce induce dedifferentiation in leaf cells
4. To increase surface area for transfers

Q. 'Cry' gene is obtained from

1. Agrobacterium tumefaciens
2. Bacillus thuringiensis
3. Rhizobium leguminosarum
4. Rhizobium phaseoli

Q. The bacteria associated with plant genetic engineering are:

1. Azotobacter agilis
2. Agrobacterium tumefaciens
3. Bacillus thuringiensis
4. Rhizobium leguminosarum

Q. Which bacteria produces Bt toxin?

Q. In the agrobacterium mediated gene transfer using the leaf disc method, what is the significance of cutting the leaf into discs?

1. To make the leaf round shape
2. To induce induce dedifferentiation in leaf cells
3. To increase surface area for transfers
4. To use both surfaces of the leaf

Q. The process in which (genetic material is carried by some external agency) viruses are involved in sexual reproduction of bacteria is called

1. Transcription
2. Transduction
3. Transformation
4. Translation

Q. Wild Ti plasmid is able induce tumors in plants due to presence of

1. Cancer genes
2. Phytohormone coding genes
3. Kanamycin resistance gene
4. Cry genes

Q. Which one of the following is used as vector for cloning genes into higher organisms?

1. Retrovirus
2. Baculovirus
3. Salmonella typhimurium
4. Rhizopus-nigricans

Q. Which one can be used as vector for transfer of genes?

1. BAC
2. Plasmid
3. YAC
4. All of the above

Q. Which among the following is a tumour inducing plasmid widely used in the production of transgenic plants?

1. Escherichia coli
2. Bacillus thuringiensis
3. Staphylococcus aureus
4. Agrobacterium tumefaciens

Q. Which of the following modifications are made to make a “tamed” Ti plasmid which can transfer cry genes to a plant’s genome ?

1. Removal of kanamycin resistance gene and adding phytohormone genes
2. Removal of phytohormone gene and adding kanamycin resistance gene
3. Removal of both kanamycin resistance gene and phytohormone genes
4. Adding both phytohormone and kanamycin resistance gene

Q. Bacillus thuringiensis strains have been used for

1. Biomineralization processes
2. Bioinsecticidal application
3. Biofertilizer application
4. Biometallurgical techniques

Q. Transfer of genetic information through transduction involves

1. Conjugation
2. Bacteriophage released from the donor cell
3. Another bacterium
4. Physical contact between donor and recipient strains

Q. Transfer of genetic material with the help of a virus is called

1. Transduction
2. Transference
3. Transformation
4. Transcription

Q. In which step the gene of interest gets incorporated in plant genome?

1. Growing the discs with shoot inducing medium
2. Growing the leaf discs with agrobacterium
3. Cutting the leaf discs
4. Growing the discs with root inducing medium

Q. The introduction of T-DNA into plants involves: