The correct option is
D Culturing of cells and study of metaphase chromosomes from amniotic fluid to identify chromosomal abnormality
Amniocentesis (also referred to as amniotic fluid test or AFT) is a medical procedure used in prenatal diagnosis of chromosomal abnormalities and foetal infections, and also used for sex determination in which a small amount of amniotic fluid, which contains foetal tissues, is sampled from the amniotic sac surrounding a developing foetus, and the foetal DNA is examined for genetic abnormalities. Amniocentesis is usually done when a woman is between 14 and 16 weeks pregnant.
So, the correct answer is 'Culturing of cells and study of metaphase chromosomes from amniotic fluid to identify chromosomal abnormality'