An analysis of chromosomal DNA using the Southern blot by hybridization technique does not use
A
PCR
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B
Electrophoresis
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C
Autoradiography
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D
Blotting
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Solution
The correct option is A PCR
Conclusion
Polymerase chain reaction or PCR is a reaction carried out in vitro to amplify a specific DNA sequence or gene of interest and create its multiple copies.
PCR is used in DNA fingerprinting only when the DNA isolated from the biological sample is in very small amounts.
Thus, it is not mandatory to perform PCR in DNA fingerprinting.