C-peptide of human insulin is :

The correct option is B Removed during the maturation of pro-insulin to insulin
In mammals, like humans, insulin is produced as a pro-hormone which needs to be processed before it becomes fully mature. It contains an extra stretch called the C peptide.
C peptide is not present in the mature insulin as it is removed during maturation of pro-insulin into insulin.

So, the correct answer is- option c.