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Restriction Enzyme

E.coli clonin...

Question

E.coli cloning vector pBR322 showing restriction sites, ori and antibiotic resistance genes is given below. Select the correct option.

A

A-Hind III,B-Eco RI, C-Bam H I, D-Sal I

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B

A-Eco RI, B-Hind III, C-Bam HI, D-Sal I

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C

A-Eco RI, B-BamH I, C-Hind III, D-Sal I

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D

None of these

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Solution

The correct option is B A-Eco RI, B-Hind III, C-Bam HI, D-Sal I
So, the correct answer is 'A-Eco RI, B- Hind III, C- Bam HI, D-Sal I'

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Similar questions

Q. Which among the following restriction endonucleases will produce blunt ends?
I. BamH I
II. Hind II
III. EcoR I
IV. Hind III
V. Sal I

Q. Match column I with column II, and choose the correct combination from the options given below.

Column I	Column II
a. Hind II	1. Recognition sequence
b. pBR $322$	2. Restriction enzyme
c. GAATTC	3. Antibiotic resistance gene
d. $a m p^{R}$	4. Cloning vector

Q. Eco RI and Rsa I restriction endonucleases require

6

4

bp sequences respectively for cleavage. In a

10 k b

DNA fragment how many probable cleavage sites are present for these enzymes

Q. There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for?

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