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Question

Enzyme Linked lmmuno Sorbent Assay is based on the principle of-
  1. Radioactive tagging
  2. Antigen – antibody interaction
  3. Autoradiography
  4. Amplification of DNA

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Solution

The correct option is B Antigen – antibody interaction
Enzyme-linked immunosorbent assay (ELISA) is a labeled immunoassay that is considered the gold standard of immunoassays. This immunological test is very sensitive and is used to detect and quantify substances, including antibodies, antigens, proteins, glycoproteins, and hormones.
ELISA detects pathogen infection by detecting the presence of antigens or by detecting the antibodies synthesised against the pathogen.

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