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Question

How is RNAi technology used to create pest resistant plants? [3]

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Solution

RNAi technology involves silencing of a specific mRNA sequence due to a complementary dsRNA molecule that binds to it and prevents translation of the mRNA (silencing). [1]

This method can be used to create pest resistance plant from pest like nematodes. Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA produced both sense and anti-sense RNA in the host cells forming a double stranded (dsRNA) that silenced the specific mRNA of the nematode which protected the plant from the parasite. [3]

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