If the DNA has following sequence then what would be sequence in newly synthesized protein from this DNA? 3'-TAC CAT GCA TGC ATG CAT GCA TGC ATT GGC-5' Template Strand 5'-ATG GTA CGT ACG TAC GTA CGT ACG TAA CCG-3' Coding Strand
A
Met – Val –Arg – Thr – Tyr – Val – Arg – Thr
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B
Val –Arg – Thr – Tyr – Val – Arg – Thr
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C
Met – Val –Arg – Thr – Tyr – Val – Arg – Thr – Asn
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D
Met - Val –Arg – Thr – Tyr – Val – Arg – Thr – Asn – Pro
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Solution
The correct option is B Val –Arg – Thr – Tyr – Val – Arg – Thr mRNA synthesized from this DNA would have sequence (Replace T with U on Coding strand): 5’ AUG GUA CGU ACG UAC GUA CGU ACG UAA CCG 3’ The start codon AUG which codes for methionine will be removed during processing of the polypeptide. GUA - Valine CGU - Arginine ACG - Threonine UAC - Tyrosine UAA isa top codon so, the translation will stop at this point. Hence, the protein will have the sequence Val –Arg – Thr – Tyr – Val – Arg – Thr