In what ways can plant tissue culture be applied for crop improvement?

Growing of cells or tissues or organs on the nutrient media to produce new plants in the laboratories in the invitro conditions is called Tissue culture. It mainly depends on the principle totipotency. Tissue culture has been exploited to create genetic variability from which crop plants can be improved, to improve the state of health of the planted material and to increase the number of desirable germplasms available to the plant breeder.. Tissue culture techniques, in combination with molecular techniques, have been successfully used to incorporate specific traits through gene transfer.