Mitochondrial DNA differs from nuclear DNA in

The correct option is B Lacking binding to histones

Mitochondrial DNA differs from Nuclear DNA as it lacks histones. This is because, mitochondria as an organelle was an independent prokaryotic cell, that during evolution became a part of the eukaryotic cell (endosymbiotic theory), where it retained its circular DNA that does not contain histones instead, is kept together by a different type of protein analogous to histone.

So, the correct answer is 'Lacking binding to histones'