Name and describe the technique that will help in solving a case of maternity dispute over the custody of a child by two different families.

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Solution

DNA fingerprinting can sort out this dispute of maternity.
Step I The technique involves southern blot hybridisation using radiolabelled VNTR as a probe.
Step II The methodology includes:
(i) DNA is isolated and digested by the restriction endonucleases.
(ii) DNA fragments are separated by electrophoresis.
(iii) Separated DNA fragments are transferred to synthetic membranes like nitrocellulase or nylon.
(iv) Hybridisation using labelled VNTR probe.
(v) Hybridised DNA fragments are detected by autoradiography.

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