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Question

Southern blotting technique uses .......... paper for embedding DNA strands.

A
Whatman's No. 1
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B
Parchment
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C
Nitrocellulose
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D
Cellophane
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Solution

The correct option is C Nitrocellulose
  • Southern blotting is used to detect specific DNA sequences in a given sample of DNA.
  • The first step in a Southern blot is to prepare the DNA mixture by breaking it into small fragments using a restriction enzyme.
  • The mixture of DNA fragments is then separated according to size by gel electrophoresis.
  • Then the double-stranded pieces of DNA are denatured into single strands within the gel.
  • Next, the DNA is transferred from the gel onto a blotting membrane called the nitrocellulose membrane.
  • The DNA is labeled using radioactive probes that can be detected by autoradiography.
    Thus the correct answer is option C.

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