Splitting pf centromere and hence separation of chromatids occurs during

The correct option is D anaphase -II

• A centromere is the DNA sequence of a chromosome that links a pair of sister chromatids.
  
• It acts as the site for the kinetochores which are highly complex multiprotein structure that is responsible for chromosome segregation.
  
• During cell division, when chromosomes are pulled apart into different cells the centromeres present in the middle of a chromosomes split.
  
• This splitting of chromosome occurs during Anaphase 2.
• During Anaphase 1- homologous chromosomes separate to each side of the cell and the centromere is intact.
• In anaphase 2- the sister chromatids separate and the centromere splits into two which result in two separate chromatids.
• The correct answer is OPTION C.