The acid used for preliminary separation of biomolecules in a living tissue is

The correct option is D Trichloroacetic acid
In order to study the different biomolecules, the living tissues are ground in trichloroacetic acid using a mortar and a pestle to form a thick soup. It is then strained to obtain two fractions: acid soluble and acid insoluble fraction. Chemicals present in both the fractions are further separated by various analytical techniques and identified.