The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

amp

^{R}

, tet

^{R}

- antibiotic resistance genes

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ori - original restriction enzyme

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rop - reduced osmotic pressure

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Hind III, EcoRI - selectable markers

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Solution

The correct option is A amp $^{R}$ , tet $^{R}$ - antibiotic resistance genes
E. coli clonning vector pBR322 shows:
Restriction sites - Hind III, EcoRI, BamHI, etc.
ori - origin of replication
Antibiotic resistance genes - amp $^{R}$ ,
tet $^{R}$
rop - for the proteins involved in the replication of the plasmid.

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The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

The correct option is A ampR, tetR - antibiotic resistance genes E. coli clonning vector pBR322 shows: Restriction sites - Hind III, EcoRI, BamHI, etc. ori - origin of replication Antibiotic resistance genes - ampR, tetR rop - for the proteins involved in the replication of the plasmid.