What are the characteristics of restriction enzymes?
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Solution
Restriction enzymes:
They are nucleases in nature.
They cleave both polynucleotide strands of DNA at a specific sequence of nitrogenous bases.
These sequences form recognition sites, restriction sites, or target sites.
These sites have palindromic sequences of nitrogenous bases.
Each palindrome has a specific endonuclease.
EcoRI is a restriction endonuclease that is found in Escherichia coli that recognizes a palindromic sequence GAATTC in both strands of DNA in the 5'-3' direction.