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Question

What are the characteristics of restriction enzymes?


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Solution

Restriction enzymes:

  1. They are nucleases in nature.
  2. They cleave both polynucleotide strands of DNA at a specific sequence of nitrogenous bases.
  3. These sequences form recognition sites, restriction sites, or target sites.
  4. These sites have palindromic sequences of nitrogenous bases.
  5. Each palindrome has a specific endonuclease.
  6. EcoRI is a restriction endonuclease that is found in Escherichia coli that recognizes a palindromic sequence GAATTC in both strands of DNA in the 5'-3' direction.

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