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Question

What will be the limit of resolution of a microscope if its numerical aperture is 0.12 and the wavelength of the light used is 6000 A0 (numerical aperture = μsin0 )?

A
305m
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B
30.5 × 107 m
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C
30.5m
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D
305mm
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Solution

The correct option is D 30.5 × 107 m

Given λ=6000A=6000×1010m=6×107m

Numerical aperture, NA=0.12

Limit of resolution is given as

X=1.22λ2NA

X=1.22×6×1072×0.12=30.5×107m

Hence the correct answer is option (B).


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