The correct option is
D Hydration of chromatin
The completion of prophase can thus be marked by the following characteristic events:
• Chromosomal material condenses and coil up to form compact mitotic chromosomes. Chromosomes are seen to be composed of two chromatids attached together at the centromere.
• Initiation of the assembly of mitotic spindle, the microtubules, the proteinaceous components of the cell cytoplasm help in the process.
Cells at the end of prophase, when viewed under the microscope, do not show golgi complexes, endoplasmic reticulum, nucleolus and the nuclear envelope.
Hydration of chromatin means uncoiling.

So, the correct answer is "option A"