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Chargaff's Rule

Which of foll...

Question

Which of following is not correct according to Chargaff's rule?

A

\frac{A}{T} = 1

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B

A + T = G + C

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C

G + A = T + C

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D

\frac{C}{G} = 1

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Solution

The correct option is C $A + T = G + C$
Erwin Chargaff did a biochemical analysis of DNA isolated from a number of organisms. Based on his biochemical analysis, he arrived at a conclusion that, in DNA, the quantity of purines is equal to the quantity of pyrimidine; Content of adenine is equal to the content of thymine and content of guanine is equal to the content of cytosine. Thus A:T ratio as well as C:G ratio will be one and the total content of A+G will be equal to the total content of T+C. But no equivalence was observed in the total content of A+T and G+C.
So, the correct answer is option B.

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Q. In the case of double-stranded DNA, which of the following base ratios are always equal to one?
a.

(A + T) / (G + C)

(A + G) / (C + T)

C / G

(G + T) / (A + C)

Select the correct answer.

Q. The following sequence contains the open reading frame of a polypeptide. How many amino acids will the polypeptide consist of?
5' A G C A T A T G A T C G T T T C T C T G C T T T G A A C T A - 3'

Q. The complementary strand for the following single strand of DNA is:

5^{'} \leftarrow A - T - G - C - T \to 3^{'}

Q. The restriction enzyme responsible for the cleavage of following sequence is
5' - G - T - C - G - A - C - 3'
3' - C - A - G - C - T - G - 5'

Q. During analysis of the DNA of an organism having

5386

nucleotides find out A

= 29 %

= 17 %

= 32 %

= 17 %

. Considering the Chargaff's rule it can be concluded that

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