Which of the following are limitations of using plasmids as vectors?

Inability to uptake large insert size

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Inability to express eukaryotic genes efficiently

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Inability to express prokaryotic genes

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Ability to uptake small insert size

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Solution

The correct option is B Inability to express eukaryotic genes efficiently
Plasmids have some limitations when used as vectors. The insert size is very small, upto 6 or 7 kb. When eukaryotic genes are inserted into plasmids, it is not efficiently expressed. To overcome these limitations, scientists have come with many new vectors such as phages, cosmids, BAC and YAC.

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