Which of the following is not true w.r.t. copy number of the linked DNA in the vector?
A
It is equal to the copy number of vector in host cell
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B
It is controlled by ori sequence
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C
It is controlled by selectable marker
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D
Depends on the sequence from where replication starts
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Solution
The correct option is C It is controlled by selectable marker ori sequence controls the copy number of the linked DNA in the vector.
Origin of replication (ori) is a sequence from where replication starts. If one wants to recover many copies of the target DNA it should be cloned in a vector whose origin supports high copy number.