Which one of the following vaccine is prepared by recombinant DNA technology ?

The correct option is A Hepatitis–B
A small piece of DNA is taken from the virus or bacterium against which we want to protect and inserted into the manufacturing cells. For example, to make the hepatitis B vaccine, part of the DNA from the hepatitis B virus is inserted into the DNA of yeast cells. Rest all are first generation.

So, the correct answer is option 'a'.